- Head of Research Group: Dr. Tamás GARAY
- Members of the Group: Gréta BÁNYAI, Nikolett DOBOS, Júlia KVASZNICZA, Afrodité NÉMETH, Marcell SZÁSZ, Adrienn VERMESSY
- Contact: garay.tamas@itk.ppke.hu
- The Laboratory of Translational Oncology was co-founded by the Department of Internal Medicine and Oncology of Semmelweis University and the Faculty of Information Technology and Bionics of Pázmány Péter Catholic University. Accordingly, this Laboratory has the unique opportunity to address questions arising in the daily clinical routine with the competences of both medical-biological and engineering approaches (bench-to-bed). Similarly, scientific results can be easily transferred into the clinics to serve as outgoing point for new scientific questions and as new therapeutic approaches (bed-to-bench).
- In the course of investigations in the Laboratory of Translational Oncology we have a comprehensive cell culture laboratory with cutting edge techniques; our aim is to identify new therapeutic targets, prognostic and predictive markers (nucleic acids and proteins, extracellular vesicles) in histological tumor samples or liquid biopsies, that could be crucial in understanding of tumorous processes. Effects of activation and inhibition of potential target molecules (e. g. on proliferation/cell cycle or the activation/inhibition of downstream signaling pathways) are tested in tumor cell models. A distinguished attention is paid to the change of migratory activity of tumor cells, as cell migration is an inevitable process during the formation of metastases.
Effect of EV, Dabrafenib (Dab), Trametinib (Tram) and combined treatment on single-cell migration. Cell migration was recorded for 24 hours, semiautomatic tracking of single cells performed using CellTracker and MSD and TTD calculated. Results of three independent measurements are shown as mean ± SEM, and p-value less than 0.05 considered as statistically significant (to vehicle: *, to Dab: #, to EV-only: §).
